sanger sequencing analysis Search Results


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Image Search Results


Workflow of analysis methods undertaken in this study. bam binary alignment map, BST bisulphite-treated, CpG cytosine-phosphate-guanine, DKD diabetic kidney disease, gDNA genomic DNA, hg human genome, NGS next generation sequencing, SNP single nucleotide polymorphism, T1D type-1 diabetes mellitus

Journal: BMC Research Notes

Article Title: Validation of differentially methylated microRNAs identified from an epigenome-wide association study; Sanger and next generation sequencing approaches

doi: 10.1186/s13104-018-3872-x

Figure Lengend Snippet: Workflow of analysis methods undertaken in this study. bam binary alignment map, BST bisulphite-treated, CpG cytosine-phosphate-guanine, DKD diabetic kidney disease, gDNA genomic DNA, hg human genome, NGS next generation sequencing, SNP single nucleotide polymorphism, T1D type-1 diabetes mellitus

Article Snippet: Regarding DNA methylation, the BST DNA Sanger sequencing analysis mirrored the methylation sites identified through the Infinium Human Methylation 450K analysis.

Techniques: Next-Generation Sequencing

Comparison of SNPs located within miR - 329 - 2 and miR - 429 identified by targeted NGS and Sanger sequencing. The data generated by both platforms showed consistent results for SNP calls. Ion PGM™ data analysed using Partek Genomics Suite is shown on the left, with the complementary Sanger sequence results shown on the right, for matching genomic DNA samples. Chr chromosome, DC diabetic control, DKD diabetic kidney disease, hg human genome, Ref reference, Seq sequence

Journal: BMC Research Notes

Article Title: Validation of differentially methylated microRNAs identified from an epigenome-wide association study; Sanger and next generation sequencing approaches

doi: 10.1186/s13104-018-3872-x

Figure Lengend Snippet: Comparison of SNPs located within miR - 329 - 2 and miR - 429 identified by targeted NGS and Sanger sequencing. The data generated by both platforms showed consistent results for SNP calls. Ion PGM™ data analysed using Partek Genomics Suite is shown on the left, with the complementary Sanger sequence results shown on the right, for matching genomic DNA samples. Chr chromosome, DC diabetic control, DKD diabetic kidney disease, hg human genome, Ref reference, Seq sequence

Article Snippet: Regarding DNA methylation, the BST DNA Sanger sequencing analysis mirrored the methylation sites identified through the Infinium Human Methylation 450K analysis.

Techniques: Comparison, Sequencing, Generated, Control